Using CRISPR To Treat Cystic Fibrosis

SKU: 135
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$109.00

ABOUT THIS PRODUCT:


In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

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Details

Group Size:

For 8 Gels

Time Required:

Complete in 45 minutes

Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.

Resources

Instructional Videos:

Biotechnology Basics:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Introduce Your Students to Gene Editing with CRISPR (featuring the EDGE™) - In the past 10 years, researchers have begun to use the CRISPR-Cas9 system to genetically modify DNA sequences. The development of this system as a gene editing tool is one of the most exciting biotechnology breakthroughs in years! In this hands-on workshop, explore CRISPR biology using the EDVOTEK® EDGE™ in a fast, easy experiment that models the development of a cure for Cystic Fibrosis.

Safety Data Sheets:

Quick Guides:

Teaching Resources:

Group Size:

For 8 Gels

Time Required:

Complete in 45 minutes

Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.

Instructional Videos:

Biotechnology Basics:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Introduce Your Students to Gene Editing with CRISPR (featuring the EDGE™) - In the past 10 years, researchers have begun to use the CRISPR-Cas9 system to genetically modify DNA sequences. The development of this system as a gene editing tool is one of the most exciting biotechnology breakthroughs in years! In this hands-on workshop, explore CRISPR biology using the EDVOTEK® EDGE™ in a fast, easy experiment that models the development of a cure for Cystic Fibrosis.

Safety Data Sheets:

Quick Guides:

Teaching Resources:

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