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Transformation of E. coli with Green Fluorescent Protein (GFP)

SKU: 223-AP08
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$95.00

ABOUT THIS PRODUCT:


In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

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Details

Group Size:

For 10 lab groups


Time Required:

Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutes


Kit Includes:

Instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes


All You Need:

Automatic micropipette (5-50 µl) and tips, two water baths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light


Storage:

Some Components Require Refrigerator and Freezer Storage


Replenisher:

Resources

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Miscellaneous:

Instructional Videos:

Group Size:

For 10 lab groups


Time Required:

Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutes


Kit Includes:

Instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes


All You Need:

Automatic micropipette (5-50 µl) and tips, two water baths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light


Storage:

Some Components Require Refrigerator and Freezer Storage


Replenisher:

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Miscellaneous:

Instructional Videos:

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