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DNA Fingerprinting Using Restriction Enzymes

SKU: 225
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$129.00

ABOUT THIS PRODUCT:


Teach your students about restriction enzyme digests in the context of forensic science! Your students will cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

• Explore DNA and restriction enzymes through the lens of forensic science
• Learn the biology, techniques, and applications of DNA fingerprinting
• Digest DNA from two suspects using the enzymes EcoRI and HindIII
• Separate and size DNA samples using gel electrophoresis
• Compare suspect and crime scene samples to determine ‘whodunnit’

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 8 gels

Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes

Kit Includes:

Instructions, “crime scene” and "suspect" Ready-to-Load™ DNA samples, DNA Standard Marker, Dryzymes® - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, SYBR® Safe stain, FlashBlue™ DNA Stain, 1 mL pipet, Microtipped Transfer Pipets, & Microcentrifuge Tubes.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, Blue Light Visualization System (use if staining with SYBR® Safe) White Light Box (use if staining with FlashBlue™), & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Instructional Videos:

BioTech Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Restriction Digests Using EDVO-Kit #225 - Dr. Danielle Snowflack demonstrates the use of restriction enzymes in your classroom with our student (and teacher) favorite experiment, EDVO-Kit #225 "DNA Fingerprinting Using Restriction Enzymes!" The history of restriction enzymes in the laboratory and tips and tricks to make your experiment a success are discussed.

Safety Data Sheets:

Quick Guides:

Replenisher

Group Size:

For 8 gels

Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes

Kit Includes:

Instructions, “crime scene” and "suspect" Ready-to-Load™ DNA samples, DNA Standard Marker, Dryzymes® - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, SYBR® Safe stain, FlashBlue™ DNA Stain, 1 mL pipet, Microtipped Transfer Pipets, & Microcentrifuge Tubes.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, Blue Light Visualization System (use if staining with SYBR® Safe) White Light Box (use if staining with FlashBlue™), & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Instructional Videos:

BioTech Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Restriction Digests Using EDVO-Kit #225 - Dr. Danielle Snowflack demonstrates the use of restriction enzymes in your classroom with our student (and teacher) favorite experiment, EDVO-Kit #225 "DNA Fingerprinting Using Restriction Enzymes!" The history of restriction enzymes in the laboratory and tips and tricks to make your experiment a success are discussed.

Safety Data Sheets:

Quick Guides:

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