Exploring Biotechnology with GFP

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Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the green fluorescent protein (GFP). The transformed cells are then grown and the GFP is purified by column chromatography. Finally, the purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis.

Group size: For 6 experiments with 4 modules each

Time required: Complete in 3 hours 15 minutes

Kit includes: instructions, BactoBeads™, plasmid DNA for GFP, IPTG, ampicillin antibiotic, calcium chloride, ReadyPour™ luria broth agar, luria broth media for recovery, petri plates, pipets, calibrated transfer pipets, inoculating loops, microtest tubes with attached caps, toothpicks, dry matrix for columns, chromatography columns, green fluorescent protein extracts, elution buffer, protein molecular weight standards, protein denaturation solution, glycerol solution, Tris-Glycine-SDS buffer, Protein Instastain

You need: incubation oven, two waterbaths, microwave or hot plate, automatic micropipet and tips, pipet pumps or bulbs, ice, long wave UV light, ring stand and clamps, lab glassware, ice, vertical gel electrophoresis apparatus and power supply, 3 Polyacrylamide Gels (12%), plastic trays or large weigh boats for optional staining & destaining, glacial acetic acid, methanol

Storage: Some Components Require Freezer Storage

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