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Transformation of E. coli with Blue and Green Fluorescent Proteins

SKU: 222
$119.00

ABOUT THIS PRODUCT:


In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

• Explore genetic engineering in this elegant and expanded experiment
• Transform E.coli with either pFluoroGreen™ or pFluoroBlue™
Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
• Expose transformed cells to IPTG to demonstrate differential gene expression
• Different plasmids help emphasize the concept of DNA>RNA>Protein>Trait

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Complete in 50 minutes and grow overnight


Kit Includes:

Instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, CaCl2, growth additive, competent cell solution, ReadyPour™ agar, recovery broth, petri plates, plastic microtipped transfer pipets, wrapped 10 mL pipet (sterile), inoculating loops (sterile), & conical tube, & microcentrifuge tubes


All You Need:

Micropipettes, two water baths, floating racks or foam tube holders, thermometer, incubation oven, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, & UV safety glasses, long wave UV light, centrifuge (optional)


Storage:

Some Components Require Refrigerator & Freezer Storage

Resources

Instructional Videos:

EdvoTech Tips:

Additional Resources:

Miscellaneous:

pFluoroGreen & pFluoroBlue Plasmid Map

Replenisher

Group Size:

For 10 lab groups


Time Required:

Complete in 50 minutes and grow overnight


Kit Includes:

Instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, CaCl2, growth additive, competent cell solution, ReadyPour™ agar, recovery broth, petri plates, plastic microtipped transfer pipets, wrapped 10 mL pipet (sterile), inoculating loops (sterile), & conical tube, & microcentrifuge tubes


All You Need:

Micropipettes, two water baths, floating racks or foam tube holders, thermometer, incubation oven, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, & UV safety glasses, long wave UV light, centrifuge (optional)


Storage:

Some Components Require Refrigerator & Freezer Storage

Instructional Videos:

EdvoTech Tips:

Additional Resources:

Miscellaneous:

pFluoroGreen & pFluoroBlue Plasmid Map