Transformation of E. coli with Blue and Green Fluorescent Proteins

SKU: 222
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$115.00

ABOUT THIS PRODUCT:


The Green Fluorescent Protein (GFP), which is responsible for bioluminescence in the jellyfish Aequorea victoria, is used extensively in all areas of science. Many organisms have been transformed with the GFP gene. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both!

DOWNLOAD SAMPLE INSTRUCTIONS
Group size: For 10 lab groups

Time required: Complete in 50 minutes and grow overnight

Kit includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ agar, Luria broth, petri dishes, sterile pipets and loops.

All you need: waterbath, 37ºC incubation oven, microwave or hot plate, long wave UV lamp.

Storage: Some Components Require Refrigerator & Freezer Storage

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