Rainbow Transformation

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Transformation is of central importance in molecular cloning since it allows for the selection, propagation, expression and purification of a gene. Positive selection for cells containing plasmid DNA is accomplished by antibiotic growth selection. In this experiment, your students will transform bacteria with a new set of rainbow color plasmids that transform non-pathogenic bacterial cells into bright, colorful cells.

Group size: For 10 lab groups

Time required: Set Up & Plating - 50 minutes / Incubation - overnight / Transformation efficiency - 15 minutes

Kit includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ Agar, Luria broth, petri dishes, sterile pipets, loops and microtubes.

All you need: waterbath, 37˚C incubation oven, microwave or hot plate

Storage: Some components require refrigerator and freezer storage

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