Cancer Gene Detection

SKU: 115
$89.00

ABOUT THIS PRODUCT:


Immortality through uncontrolled cell division is a characteristic of cancer cells. The p53 gene is a tumor suppressor gene which prevents this. Mutations in this gene are present in more than 50% of cancers. Testing people for mutations in their p53 gene can indicate an increased risk in developing cancer. These tests raise intriguing ethical questions for both the individual tested and the family of an individual who chooses to be tested. In this experiment, students determine a pedigree for a family suspected to be carriers of mutations in their p53 genes. A DNA test indicates their likelihood of developing cancer.

• Discover the molecular biology behind familial cancers
• Explore how oncologists use pedigrees and molecular diagnostics
• Construct a family’s pedigree for Li-Fraumeni syndrome
• Perform agarose gel electrophoresis to separate different sized DNA molecules
• Load, run, analyze, and size control samples and a patient’s blood, tumor, and tissue samples to identify mutations in their p53 gene

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group size:

For 8 Gels

Time required:

Complete in 45 minutes

Kit includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets.

All you need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Edvotek at Home:

Cancer Gene Detection:In this experiment, students will gain an understanding of the p53 tumor suppressor gene and its role in familial cancers. Students will learn about Restriction Fragment Length Polymorphism (RFLP) analysis and separation of DNA fragments using agarose gel electrophoresis. At the end of the activity, students will have experience analyzing observed results and be capable of transforming the abstract concepts of restriction digestion and electrophoresis into an enhanced scientific understanding.

Group size:

For 8 Gels

Time required:

Complete in 45 minutes

Kit includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets.

All you need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Edvotek at Home:

Cancer Gene Detection:In this experiment, students will gain an understanding of the p53 tumor suppressor gene and its role in familial cancers. Students will learn about Restriction Fragment Length Polymorphism (RFLP) analysis and separation of DNA fragments using agarose gel electrophoresis. At the end of the activity, students will have experience analyzing observed results and be capable of transforming the abstract concepts of restriction digestion and electrophoresis into an enhanced scientific understanding.
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