In Search of the Cancer Gene

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Suppressor genes such as p53 are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations in genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation. This experiment does not contain human DNA.

Group size: For 6 lab groups

Time required: Complete in 60 minutes

Kit includes: instructions, Ready-to-load™ Predigested DNA samples, UltraSpec-Agarose™ powder, electrophoresis buffer, InstaStain® Ethidium Bromide, 5 autoradiograms.

All you need: electrophoresis apparatus & power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath (65ºC), UV Transilluminator, pipet pump or bulb, 250 ml Flasks, distilled or deionized water.

Storage: Refrigerator

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