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AIDS Kit I: Simulation of HIV Detection by ELISA

SKU: 271
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$125.00

ABOUT THIS PRODUCT:


An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a color change upon addition of substrate. In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

• Learn the biology and methodology of the Enzyme-Linked Immunosorbent Assay (ELISA)
• Perform an indirect (two-step) ELISA with controls and patient samples
• Experiment replicates a clinical screen to detect HIV antibodies in patient blood samples
• Understand the molecular biology of HIV and the pathogenesis of AIDS

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Complete in 60 minutes


Kit Includes:

Instructions, 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary antibody (lyophilized), secondary antibody (lyophilized), ABTS (lyophilized), ABTS reaction buffer, microtiter plates, transfer pipets, snap-top microcentrifuge tubes, 15 mL conical tubes


All You Need:

Distilled or deionized water, beakers, disposable lab gloves, safety goggles, automatic micropipettes (50µl) and tips (recommended)


Storage:

Refrigerator

Resources

Instructional Videos:

Edvotek® YouTube Live Stream - Teaching the ELISA Using Public Health - HIV Biology an Detection - In this workshop, Dr. Danielle Snowflack discusses the HIV virus using Origami Organelles, the ways clinicians test for the virus using Edvotek's best-selling ELISA experiment Cat. #271 AIDS Kit I: Simulation of HIV Detection by ELISA, and the methods that public health professionals use to trace the spread of the virus.

Safety Data Sheets:

Replenisher

Group Size:

For 10 lab groups


Time Required:

Complete in 60 minutes


Kit Includes:

Instructions, 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary antibody (lyophilized), secondary antibody (lyophilized), ABTS (lyophilized), ABTS reaction buffer, microtiter plates, transfer pipets, snap-top microcentrifuge tubes, 15 mL conical tubes


All You Need:

Distilled or deionized water, beakers, disposable lab gloves, safety goggles, automatic micropipettes (50µl) and tips (recommended)


Storage:

Refrigerator

Instructional Videos:

Edvotek® YouTube Live Stream - Teaching the ELISA Using Public Health - HIV Biology an Detection - In this workshop, Dr. Danielle Snowflack discusses the HIV virus using Origami Organelles, the ways clinicians test for the virus using Edvotek's best-selling ELISA experiment Cat. #271 AIDS Kit I: Simulation of HIV Detection by ELISA, and the methods that public health professionals use to trace the spread of the virus.

Safety Data Sheets: