• Home
  • Code Breakers: Using CRISPR to Rewrite Genetics

Code Breakers: Using CRISPR to Rewrite Genetics

SKU: 307
NEW
$275.00

ABOUT THIS PRODUCT:

Unlock the exciting world of gene editing and investigate the incredible power of CRISPR technology right from your classroom! In this experiment, students explore cutting-edge genetic engineering using CRISPR-Cas9 to knock out GFP and B-galactosidase genes in classroom safe bacteria. Additionally, students test the specificity of CRISPR-Cas9 first hand by switching the CRISPR RNA templates and analyzing the results. 

• Explore genetic engineering, CRISPR, and the central dogma of molecular biology in this comprehensive experiment.
• Transform classroom-safe E. coli with CRISPR RNA templates to test the targeting specificity of the technology.
• Identify successful gene knockout based on the color of the cells. 

 

Details

Group Size:

For 10 Lab Groups


Time Required:

Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutes


Kit Includes:

Instructions, BactoBeads™ E. coli GFP Host and B-gal host, CRISPR RNA template plasmid DNA, ampicillin, IPTG, X-Gal, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes


All You Need:

Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves


Storage:

Some Components Require Refrigerator and Freezer Storage Upon Receipt.

Resources

Teaching Resources:

Group Size:

For 10 Lab Groups


Time Required:

Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutes


Kit Includes:

Instructions, BactoBeads™ E. coli GFP Host and B-gal host, CRISPR RNA template plasmid DNA, ampicillin, IPTG, X-Gal, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes


All You Need:

Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves


Storage:

Some Components Require Refrigerator and Freezer Storage Upon Receipt.