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Sep. of RNA and DNA by Gel Filtration Chromatography

SKU: 204
$125.00

ABOUT THIS PRODUCT:


Gel filtration chromatography separates molecules on the basis of size and shape. This experiment provides a LyphoSample™ mixture of RNA and DNA that is separated on a gel exclusion column. The purified fractions of DNA and RNA are analyzed by agarose gel electrophoresis.

• Introduce the principles and practice of gel filtration chromatography
• Separate DNA from RNA and then visualize these two molecules
• Learn to pack a column, collect fractions, perform electrophoresis, and stain DNA & RNA
• Compare and contrast molecule separation by chromatography and electrophoresis

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 5 separations and 5 gels


Time Required:

Complete in 90 minutes


Kit Includes:

Instructions, DNA/RNA LyphoSample™, Dry matrix, elution buffer, chromatography columns, agarose, gel loading solution, buffer, InstaStain® Blue and FlashBlue™ stain.


All You Need:

Electrophoresis apparatus, power supply, ring stands with clamps, automatic micropipette with tips, microwave or hot plate, pipet pumps or bulbs, 5 or 10 ml pipets, balance, lab glassware, weigh boats, white light visualization system, photodocumentation system (optional), distilled water.


Storage:

Refrigerator

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Group Size:

For 5 separations and 5 gels


Time Required:

Complete in 90 minutes


Kit Includes:

Instructions, DNA/RNA LyphoSample™, Dry matrix, elution buffer, chromatography columns, agarose, gel loading solution, buffer, InstaStain® Blue and FlashBlue™ stain.


All You Need:

Electrophoresis apparatus, power supply, ring stands with clamps, automatic micropipette with tips, microwave or hot plate, pipet pumps or bulbs, 5 or 10 ml pipets, balance, lab glassware, weigh boats, white light visualization system, photodocumentation system (optional), distilled water.


Storage:

Refrigerator

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides: