Principles of Gel Filtration Chromatography

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Introduce chromatographic separation to your class and show them how dyes of different colors separate on the basis of their size and shape. This experiment contains materials for dye separation which include dye sample, elution buffer and plastic disposables. Columns may be rinsed and reused.

Group size: For 10 lab groups

Time required: Packing Column 20 minutes / Column Separation 40 minutes

Kit includes: instructions, sample mixture, chromatography columns, dry matrix, elution buffer, transfer pipets, microtest tubes.

You need: 50 or 100 ml beakers, 25 ml beaker or test tube, ring stands with clamps, distilled water.

Storage: Room Temperature

Safety Data Sheets:

Instructional Videos:

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