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Affinity Chromatography of Glucose Binding Proteins

SKU: 277
$139.00

ABOUT THIS PRODUCT:


In this experiment, students will prepare a seed extract from Jack Bean Meal, fractionate the extract by affinity chromatography, and elute the bound glucose binding protein. The presence of biological activity is determined by an immunoblot enzyme assay.

• Explore proteins and learn the principle of affinity chromatography
• Work your way through three connected experiments
• Carry out a simple protein extraction
• Use column chromatography to isolate a carbohydrate-binding lectic
• Perform an immunoblot enzyme assay on different elations

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Details

Group Size:

For 10 lab groups

Time Required:

Requires 2 hours

Kit Includes:

Instructions, affinity gel, jack bean meal, various solutions and buffers, petri plates, columns with tips, conical tubes, membranes, and transfer pipets.

All You Need:

Clinical centrifuge, vortex or shaking platform, micropipet and tips, ring stands and clamps, lab glassware, pipets & pumps, microtest tubes, forceps, water.

Storage:

Some Components Require Refrigerator Storage

Resources

Instructional Videos:

Safety Data Sheets:

Printable Word Puzzles:

Replenisher

Group Size:

For 10 lab groups

Time Required:

Requires 2 hours

Kit Includes:

Instructions, affinity gel, jack bean meal, various solutions and buffers, petri plates, columns with tips, conical tubes, membranes, and transfer pipets.

All You Need:

Clinical centrifuge, vortex or shaking platform, micropipet and tips, ring stands and clamps, lab glassware, pipets & pumps, microtest tubes, forceps, water.

Storage:

Some Components Require Refrigerator Storage

Instructional Videos:

Safety Data Sheets:

Printable Word Puzzles:

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