Quantitative ELISA

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Now with NEW substrate! Antibodies are highly specific in their recognition of antigens. This ELISA experiment demonstrates the quantitation of varying concentrations of viral antigens as detected by the intensity of the color reaction due to the accumulation of products. This laboratory activity meets the requirements in the BSCS Blue Biology curriculum.

Group size: For 6 lab groups

Time required: Complete in 2 hours

Kit includes: instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipets.

All you need: distilled or deionized water, 37º C incubation oven, automatic micropipets with tips, laboratory glassware.

Storage: Some Components Require Refrigerator Storage.

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