Quantitative ELISA

SKU: 278
$125.00

ABOUT THIS PRODUCT:


Now with NEW substrate! Antibodies are highly specific in their recognition of antigens. This ELISA experiment demonstrates the quantitation of varying concentrations of viral antigens as detected by the intensity of the color reaction due to the accumulation of products. This laboratory activity meets the requirements in the BSCS Blue Biology curriculum.

• Master the Enzyme-Linked Immunosorbent Assay (ELISA) with this advanced experiment
• Prepare serial dilutions and perform ELISAs to determine the concentration of two antigens
• Explore the specificity of ELISAs by testing multiple antigen-antibody combinations
• Quantify results using imaging and graphing software

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Complete in 2 hours


Kit Includes:

Instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipets.


All You Need:

Distilled or deionized water, 37º C incubation oven, automatic micropipettes with tips, laboratory glassware.


Storage:

Some Components Require Refrigerator Storage

Resources

Instructional Videos:

Safety Data Sheets:

Replenisher

Group Size:

For 10 lab groups


Time Required:

Complete in 2 hours


Kit Includes:

Instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipets.


All You Need:

Distilled or deionized water, 37º C incubation oven, automatic micropipettes with tips, laboratory glassware.


Storage:

Some Components Require Refrigerator Storage

Instructional Videos:

Safety Data Sheets: