Ion Exchange Chromatography

SKU: 243
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$115.00

ABOUT THIS PRODUCT:


Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

• Understand and practice ion exchange chromatography
• Explore how charge affects a molecule’s behavior
• Prepare a column then alter its pH to isolate different molecules
• Specially formulated dyes allow for easy visualization and for (optional) quantification using a spectrophotometer

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Complete in 60-90 minutes


Kit Includes:

Instructions, ion exchanger, potassium acetate buffer, blue and red dyes, chromatography columns, assorted pipets and tubes.


You Need:

Spectrophotometer; cuvettes, ring stands and clamps, test tubes, lab glassware, distilled water, 5 ml pipets and pumps.


Storage:

Room Temperature


Resources

Instructional Videos:

Safety Data Sheets:

YouTube Live Stream:

Printable Fun Activities:

Group Size:

For 10 lab groups


Time Required:

Complete in 60-90 minutes


Kit Includes:

Instructions, ion exchanger, potassium acetate buffer, blue and red dyes, chromatography columns, assorted pipets and tubes.


You Need:

Spectrophotometer; cuvettes, ring stands and clamps, test tubes, lab glassware, distilled water, 5 ml pipets and pumps.


Storage:

Room Temperature


Instructional Videos:

Safety Data Sheets:

YouTube Live Stream:

Printable Fun Activities: