Ion Exchange Chromatography

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Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Group size: For 10 lab groups

Time required: Complete in 60-90 minutes

Kit includes: instructions, ion exchanger, potassium acetate buffer, blue and red dyes, chromatography columns, assorted pipets and tubes.

You need: spectrophotometer; cuvettes, ring stands and clamps, test tubes, lab glassware, distilled water, 5 ml pipets and pumps.

Storage: Room Temperature

Safety Data Sheets:

Instructional Videos:

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