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In Search of the Sickle Cell Gene by Southern Blot

SKU: 315
$179.00

ABOUT THIS PRODUCT:


Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

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Details

Group Size:

For 5 lab groups

Time Required:

Electrophoresis - 45 minutes | Blot - overnight | Stain & Destain - 10 minutes

Kit Includes:

Instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.

All You Need:

Electrophoresis apparatus, power supply, microwave or hot plate, water bath and 80ºC incubation oven.

Storage:

Some Components Require Freezer Storage

Resources

Instructional Videos:

Safety Data Sheets:

Replenisher

Group Size:

For 5 lab groups

Time Required:

Electrophoresis - 45 minutes | Blot - overnight | Stain & Destain - 10 minutes

Kit Includes:

Instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.

All You Need:

Electrophoresis apparatus, power supply, microwave or hot plate, water bath and 80ºC incubation oven.

Storage:

Some Components Require Freezer Storage

Instructional Videos:

Safety Data Sheets:

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