In Search of the Sickle Cell Gene by Southern Blot

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Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

Group size: For 5 lab groups

Time required: Electrophoresis - 45 minutes | Blot - overnight | Stain & DeStain - 10 minutes

Kit includes: instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.

All you need: electrophoresis apparatus, power supply, microwave or hot plate, waterbath and 80ºC incubation oven.

Storage: Some Components Require Freezer Storage

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