Southern Blot Analysis

SKU: 207
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$115.00

ABOUT THIS PRODUCT:


This experiment introduces your students to Southern blotting as a tool for "DNA Fingerprinting" in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

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Details

Group Size:

For 5 lab groups


Time Required:

Electrophoresis - 45 minutes / Blot - overnight / Stain/Destain - 10 minutes


Kit Includes:

Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.


All You Need:

Electrophoresis apparatus, power supply, 65º C Water bath, DNA visualization system, staining net & tray, automatic micropipettes, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps


Replenisher:

Group Size:

For 5 lab groups


Time Required:

Electrophoresis - 45 minutes / Blot - overnight / Stain/Destain - 10 minutes


Kit Includes:

Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.


All You Need:

Electrophoresis apparatus, power supply, 65º C Water bath, DNA visualization system, staining net & tray, automatic micropipettes, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps


Replenisher:

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