LAB 2: Principles of Enzyme Catalysis

SKU: 282
$129.00

ABOUT THIS PRODUCT:


This easy and safe experiment allow your students to learn about enzyme catalysis, the nature of enzyme action and protein structure-function relationships. Students will perform an enzyme assay and determine the rate of the enzymatic reaction. This experiment uses a safer system that eliminates the need for sulfuric acid and potassium permanganate.

• Explore enzymes and protein structure-function relationships
• Experiment with the enzyme catalase ad observe it catalyzes the decomposition of hydrogen peroxide
• Quantify color changes using a spectrophotometer and determine the rate of the reaction
• Ideal for the classroom: safe, colorful, and perfectly paced for both easy observation and quick results

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Complete in 30-45 minutes


Kit Includes:

Instructions, catalase solution, 1.2% hydrogen peroxide, phosphate buffer, assay reagent, acidification solution, color enhancer, color developer.


All You Need:

Visible wavelength spectrophotometer, timer, pipet pumps, test tubes & racks, 1, 5 & 10 ml pipets, beakers, graph paper, distilled water, ice.


Storage:

Some Components Require Refrigerator & Freezer Storage

Resources

Replenisher

Group Size:

For 10 lab groups


Time Required:

Complete in 30-45 minutes


Kit Includes:

Instructions, catalase solution, 1.2% hydrogen peroxide, phosphate buffer, assay reagent, acidification solution, color enhancer, color developer.


All You Need:

Visible wavelength spectrophotometer, timer, pipet pumps, test tubes & racks, 1, 5 & 10 ml pipets, beakers, graph paper, distilled water, ice.


Storage:

Some Components Require Refrigerator & Freezer Storage