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Bee-coming Royals: DNA Methylation of Honey Bees

SKU: 205
NEW
$159.00

ABOUT THIS PRODUCT:


In this experiment, students explore the effects of DNA methylation on restriction enzyme activity to determine whether samples belong to worker bee or queen bee larvae.

• Learn how DNA methylation can influence gene expression and its importance in determining the developmental fate of organisms, such as the differentiation between worker and queen bee larvae
• Gain hands-on experience in interpreting gel electrophoresis results to draw conclusions about the methylation status
• Explore how DNA methylation affects the ability of restriction enzymes to cut DNA
• Meets NGSS HS DCI LS1-1, LS3-1, LS3-2, through the investigation of how variations in DNA methylation can lead to differences in physical traits and behaviors

* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas).

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

6 sets of restriction digestions

Time Required:

Complete in under 2 hours and 30 minutes

Kit Includes:

Instructions, Bee samples, Restriction Digest Reaction Buffer, EdvoQuick™ DNA Ladder, DpnI and DpnII Restriction Enzymes, Restriction Enzyme Dilution Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe DNA Stain, & Microcentrifuge Tubes

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Blue Light Transilluminator (for staining with SYBR® Safe DNA Stain), Water Bath, & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Replenisher

Group Size:

6 sets of restriction digestions

Time Required:

Complete in under 2 hours and 30 minutes

Kit Includes:

Instructions, Bee samples, Restriction Digest Reaction Buffer, EdvoQuick™ DNA Ladder, DpnI and DpnII Restriction Enzymes, Restriction Enzyme Dilution Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe DNA Stain, & Microcentrifuge Tubes

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Blue Light Transilluminator (for staining with SYBR® Safe DNA Stain), Water Bath, & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

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