Analysis of DNA Methylation Using Restriction Enzymes

SKU: 205
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$139.00

ABOUT THIS PRODUCT:


In this experiment, students explore the effects of DNA methylation on restriction enzyme activity. Plasmid DNA will be digested with the restriction enzymes DpnI and DpnII. When digested with these enzymes, methylated and unmethylated DNA will produce restriction fragments that are distinct from one another. The restriction fragments are then analyzed using agarose gel electrophoresis. After visualizing the gel, students determine which sample is methylated.

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Details

Group Size:

6 sets of restriction digestions


Time Required:

Complete in under 2 hours and 30 minutes


Kit Includes:

Instructions, methylated and unmethylated Plasmid DNA, EdvoQuick™ DNA Ladder, DpnI and DpnII Restriction Enzymes, Restriction Enzyme Dilution Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), InstaStain® Ethidium Bromide, & Microcentrifuge Tubes


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, UV Transilluminator (for staining with Ethidium Bromide), Water Bath, & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt


Replenisher:

Group Size:

6 sets of restriction digestions


Time Required:

Complete in under 2 hours and 30 minutes


Kit Includes:

Instructions, methylated and unmethylated Plasmid DNA, EdvoQuick™ DNA Ladder, DpnI and DpnII Restriction Enzymes, Restriction Enzyme Dilution Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), InstaStain® Ethidium Bromide, & Microcentrifuge Tubes


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, UV Transilluminator (for staining with Ethidium Bromide), Water Bath, & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt


Replenisher:

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