AIDS Kit III: Simulation of HIV Detection by Protein Electrophoresis

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The Human Immunodeficiency Virus (HIV) causes acquired immune deficiency syndrome (AIDS), a serious disease that suppresses a patient’s immune system which leaves them susceptible to infections. In this experiment, students will use SDS-PAGE to simulate the identification of HIV proteins in simulated patient samples. The results of this test are used to diagnose an HIV infection.

Group size: For 6 lab groups

Time required: Electrophoresis - 60 minutes / Staining/Destaining - optional, 2 hours

Kit Includes: instructions, denatured LyphoProtein™ samples, standard protein markers, practice gel loading solution, buffer, transfer pipets, Protein InstaStain®.

All You Need: 3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, aluminum foil or foam waterbath float, white light visualization system, automatic micropipet with fine tips, microcentrifuge tube holder, lab glassware, methanol, glacial acetic acid, glass tray, plastic wrap, and distilled or deionized water.

Storage: Some Components Require Freezer Storage

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