AIDS Kit III: Simulation of HIV Detection by Protein Electrophoresis

SKU: 151
$95.00

ABOUT THIS PRODUCT:


The Human Immunodeficiency Virus (HIV) causes acquired immune deficiency syndrome (AIDS), a serious disease that suppresses a patient’s immune system which leaves them susceptible to infections. In this experiment, students will use SDS-PAGE to simulate the identification of HIV proteins in simulated patient samples. The results of this test are used to diagnose an HIV infection.

• Explore protein diversity and analysis using polyacrylamide gel electrophoresis (PAGE)
• Examine the structure of HIV and key proteins critical to its replication
• Perform a PAGE clinical screen to identify HIV proteins in simulated blood samples and diagnose three patients.
• Skip the long and messy staining step with our pre-stained protein samples!

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 6 lab groups

Time Required:

Electrophoresis - 60 minutes / Staining/Destaining - optional, 2 hours

Kit Includes:

Instructions, denatured LyphoProtein™ samples, standard protein markers, practice gel loading solution, buffer, transfer pipets, Protein InstaStain®.

All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, aluminum foil or foam water bath float, white light visualization system, automatic micropipette with fine tips, microcentrifuge tube holder, lab glassware, methanol, glacial acetic acid, glass tray, plastic wrap, and distilled or deionized water.

Storage:

Some Components Require Freezer Storage

Resources

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Protein Electrophoresis - In this workshop, Dr. Danielle Snowflack runs a protein electrophoresis experiment from start to finish. While the gel is running, she discusses how proteins are built within a cell and how their structure affects their function.

Additional Resources:

Replenisher

Group Size:

For 6 lab groups

Time Required:

Electrophoresis - 60 minutes / Staining/Destaining - optional, 2 hours

Kit Includes:

Instructions, denatured LyphoProtein™ samples, standard protein markers, practice gel loading solution, buffer, transfer pipets, Protein InstaStain®.

All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, aluminum foil or foam water bath float, white light visualization system, automatic micropipette with fine tips, microcentrifuge tube holder, lab glassware, methanol, glacial acetic acid, glass tray, plastic wrap, and distilled or deionized water.

Storage:

Some Components Require Freezer Storage

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Protein Electrophoresis - In this workshop, Dr. Danielle Snowflack runs a protein electrophoresis experiment from start to finish. While the gel is running, she discusses how proteins are built within a cell and how their structure affects their function.

Additional Resources:

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