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  • Using CRISPR To Treat Cystic Fibrosis with SYBR® Safe

Using CRISPR To Treat Cystic Fibrosis with SYBR® Safe

SKU: 135-S
$109.00

ABOUT THIS PRODUCT:


In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

**Includes SYBR® Safe DNA Stain, NOT FlashBlue™ DNA Stain**

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:


For 8 Gels



Time Required:


Complete in 45 minutes



Kit Includes:


Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.



Storage:


Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.


Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Group Size:


For 8 Gels



Time Required:


Complete in 45 minutes



Kit Includes:


Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.



Storage:


Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.


Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides: