PRODUCT UPDATE:
Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.
Group Size:
For 5 lab groups
Time Required:
Complete three modules in 3 hours 10 minutes
Kit includes:
Instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, BactoBeads™ for transformation, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies
You Need:
Incubation oven, two water baths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice.
Storage:
Some Components Require Refrigerator and Freezer Storage
Instructional Videos:
BioTech Equipment Tips:
Edvotek Informational Video:
Safety Data Sheets:
Miscellaneous:
Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.
Group Size:
For 5 lab groups
Time Required:
Complete three modules in 3 hours 10 minutes
Kit includes:
Instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, BactoBeads™ for transformation, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies
You Need:
Incubation oven, two water baths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice.
Storage:
Some Components Require Refrigerator and Freezer Storage
Instructional Videos:
BioTech Equipment Tips:
Edvotek Informational Video:
Safety Data Sheets:
Miscellaneous: