Group size: For 5 lab groups
Time required: Complete three modules in 3 hours 10 minutes
Kit includes: instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, BactoBeads™ for transformation, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies
You need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice.
Storage: Some Components Require Refrigerator and Freezer Storage