LAB 4: Plant Pigment Chromatography and Photosynthesis

SKU: 284
$129.00

ABOUT THIS PRODUCT:


Chromatographic separation is performed by thin layer chromatography. Key reagents are included to demonstrate photosynthesis.

• Understand and explore photosynthesis using biotechnology
• Separate chloroplast pigments using thin-layer chromatography
• Identify key pigments then measure and calculate their Rfs
• Observe photosynthesis’ light dependent reactions using the color-changing dye DPIP
• Perform a chromogenic assay to investigate factors that affect photosynthetic rate

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Requires 1 hour 45 minutes (2 Lab Periods)


Kit Includes:

Instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.


All You Need:

Visible wavelength spectrophotometer, clinical benchtop centrifuge, blender, flood light, calculator, timer, test tubes; racks, beakers, 1 & 5 ml pipets, pipet pumps, metric rulers, cheesecloth, 95-100% isopropanol, distilled water, fresh spinach, ice.


Storage:

Some Components Require Freezer Storage

Resources

Replenisher

Group Size:

For 10 lab groups


Time Required:

Requires 1 hour 45 minutes (2 Lab Periods)


Kit Includes:

Instructions, chloroplast extraction; dilution buffers, reducing reagent, DCIP, standard pigments, chromatography solvent, Sarkosyl, thin layer cellulose chromatography plate, microliter capillary pipets, tubes and transfer pipets.


All You Need:

Visible wavelength spectrophotometer, clinical benchtop centrifuge, blender, flood light, calculator, timer, test tubes; racks, beakers, 1 & 5 ml pipets, pipet pumps, metric rulers, cheesecloth, 95-100% isopropanol, distilled water, fresh spinach, ice.


Storage:

Some Components Require Freezer Storage