What's In My Lunch? Quantitative Milk Allergy ELISA

SKU: 266
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NGSS-aligned with MS-LS1

Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods. In this inquiry-based experiment, students will master the concepts behind the enzyme-linked immunosorbent assay (ELISA). Students will perform an ELISA to detect the presence and measure the concentration of whey protein in various food products.

Group size: For 10 lab groups

Time required: Complete in 2 horus

Kit includes: instructions, 10X Dilution Buffer, Whey Antigen, 10X PBST (Wash Buffer), Primary Antibody, Secondary Antibody, Aminosalicylic Acid, Hydrogen Peroxide, microtiter plates, snap-top microcentrifuge tubes, homogenization pestles with tubes, 15 & 50 ml conical tubes, transfer pipets

All you need: Various food samples to be tested, distilled or deionized water, beakers or flasks, paper towels, 37˚C incubator, disposable lab gloves, safety goggles, automatic micropipets (5-50µl, 100-1000µl) and tips, digital camera or cell phone with camera (optional), computers with internet, image analysis program, and graphing program (optional)

Storage: Some Components Require Refrigerator Storage

Safety Data Sheets:

Instructional Videos:

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