What's In My Lunch? Quantitative Milk Allergy ELISA

$139.00

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ABOUT THIS PRODUCT:

NGSS-aligned with MS-LS1

Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods. In this inquiry-based experiment, students will master the concepts behind the enzyme-linked immunosorbent assay (ELISA). Students will perform an ELISA to detect the presence and measure the concentration of whey protein in various food products.

• Explore enzyme-linked immunosorbent assays (ELISAs) and the immune system
• Perform an ELISA on food samples of your choice
• Detect the milk protein whey and determine its concentration
• Learn how the food industry uses biotechnologies to detect allergens
• A strong STEM lab! Make serial dilutions, use computer imaging technologies, and create a standard curve

DOWNLOAD SAMPLE INSTRUCTIONS

Details

Group Size:

For 10 lab groups

Time Required:

Complete in 2 horus

Kit Includes:

Instructions, 10x ELISA wash buffer, ELISA dilution buffer, whey antigen (lyophilized), anti-whey antibody (lyophilized), secondary antibody, TMB substrate, stop solution, strip tubes (8-well), snap-top microcentrifuge tubes, homogenization pestles with tubes, 15 mL conical tubes, & transfer pipets

All You Need:

Various food samples to be tested, distilled or deionized water, beakers or flasks, paper towels, disposable lab gloves, safety goggles, automatic micropipettes (5-50µl, 100-1000µl) and tips, digital camera or cell phone with camera (optional), computers with internet, image analysis program, and graphing program (optional)

Storage:

Some Components Require Refrigerator Storage

Resources

Instructional Videos: