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Transformation of E. coli with pGAL™ (Blue Colony)™

SKU: 221
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$119.00

ABOUT THIS PRODUCT:


In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

No IPTG used in this experiment.

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Details

Group Size:

For 10 lab groups


Time Required:

Complete in 50 minutes and grow overnight


Kit Includes:

Instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.


All You Need:

Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves


Storage:

Some Components Require Refrigerator and Freezer Storage


Replenisher:

Resources

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Miscellaneous:

Instructional Videos:

Group Size:

For 10 lab groups


Time Required:

Complete in 50 minutes and grow overnight


Kit Includes:

Instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.


All You Need:

Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves


Storage:

Some Components Require Refrigerator and Freezer Storage


Replenisher:

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Miscellaneous:

Instructional Videos:

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