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Cleavage of Lambda DNA with Eco RI Restriction Enzyme

SKU: 212
$119.00

ABOUT THIS PRODUCT:


The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.

• Introduce restriction enzymes and their specificity
• Prepare control and EcoRI reactions of lambda phage DNA
• Use electrophoresis to visualize and size uncut and digested samples
• Explore restriction enzyme mapping

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 restriction digestions and 5 gels


Time Required:

Complete in 90 minutes


Kit Includes:

Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK® Enzyme Grade Water, Eco RI Dryzymes™ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, White Light Box, & Microwave or Hot Plate.


Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Replenisher

Group Size:

For 10 restriction digestions and 5 gels


Time Required:

Complete in 90 minutes


Kit Includes:

Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK® Enzyme Grade Water, Eco RI Dryzymes™ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, White Light Box, & Microwave or Hot Plate.


Storage:

Some Components Require Freezer Storage Upon Receipt

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides: