Restriction Enzyme Mapping

SKU: 206
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$115.00

ABOUT THIS PRODUCT:


In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.

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Details

Group Size:

6 sets of restriction digestions


Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes


Kit Includes:

Instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt


Replenisher:

Group Size:

6 sets of restriction digestions


Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes


Kit Includes:

Instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt


Replenisher:

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