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Restriction Enzyme Mapping

SKU: 206
$119.00

ABOUT THIS PRODUCT:


In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.

• Explore restriction enzymes, plasmid DNA, and genetic mapping
• Perform four DNA digestions and analyze the results using gel electrophoresis
• Create a standard curve, calculate the lengths of DNA fragments, and construct an enzyme map of the original plasmid
• A restriction enzyme lab with a strong analysis component!

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

6 sets of restriction digestions


Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes


Kit Includes:

Instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Replenisher

Group Size:

6 sets of restriction digestions


Time Required:

Complete in 1 hour 20 minutes to 1 hour 45 minutes


Kit Includes:

Instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.


Storage:

Some Components Require Freezer Storage Upon Receipt

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides: