Isolation of E. coli Chromosomal DNA

SKU: 203
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$119.00

ABOUT THIS PRODUCT:


Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.

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Details

Group Size:

For 20 DNA isolations and 5 gels


Time Required:

Complete in 1 hour 45 minutes


Kit Includes:

Instructions, Chromosomal LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.


All You Need:

Water bath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol.


For Optional Electrophoresis:

Electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system.


Storage:

Room Temperature

Group Size:

For 20 DNA isolations and 5 gels


Time Required:

Complete in 1 hour 45 minutes


Kit Includes:

Instructions, Chromosomal LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.


All You Need:

Water bath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol.


For Optional Electrophoresis:

Electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system.


Storage:

Room Temperature

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