Isolation of E. coli Chromosomal DNA

$139.00

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ABOUT THIS PRODUCT:

Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.

• Discover and understand DNA extraction and visualization
• Isolate high molecular weight chromosomal DNA from our bacteria LyphoCells™
• Visualize by spooling and by gel electrophoresis
• Gel results allow students to evaluate extraction success
• Suitable for both small (10 groups) and large (20 groups) classes

DOWNLOAD SAMPLE INSTRUCTIONS

Details

Group Size:

For 20 DNA isolations and 5 gels

Time Required:

Complete in 1 hour 45 minutes

Kit Includes:

Instructions, Chromosomal LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.

All You Need:

Water bath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol.

For Optional Electrophoresis:

Electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system.

Storage:

Room Temperature

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides: