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PCR Amplification of DNA

SKU: 330
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In this easy PCR experiment, students will make billions of copies of a small amount of DNA in just 90 minutes! They will just need to mix template DNA & primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students will see the increasing amounts of DNA for themselves, taking samples every few cycles and analyzing them on a DNA gel.

PRODUCT UPDATE: This kit includes the new LyphoTemplate™ and LyphoPrimer™. Update effective for all #330 kits purchased after November 7, 2016. Please read accompanying literature for your kit.

Group size: For 10 lab groups

Time required:

PCR Setup - 10 minutes
PCR - 90 minutes
Electrophoresis - 30 minutes
Staining - 5 minutes to overnight

Kit includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, DNA Standard Marker, Ultra-pure Water, LyphoTemplate™, TE Buffer, UltraSpec-Agarose™, 50X Electrophoresis Buffer, FlashBlue™ Stain, SYBR® Safe DNA Stain, Microcentrifuge & PCR Tubes.

All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box, (3) Water Baths (if performing experiment without a Thermal Cycler), Microcentrifuge & Microwave or Hot plate.

Storage: Some Components Require Freezer Storage Upon Receipt.

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