Determination of Protein Molecular Weight

SKU: 153
$75.00

ABOUT THIS PRODUCT:


Using prestained LyphoProteins™, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained Proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.

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Details

Groups Size:

For 6 groups sharing 3 gels


Time Required:

Electrophoresis - 60 minutes / Staining - 20 minutes / Destaining - 2 hours


Kit Includes:

Instructions, denatured LyphoProtein™ samples, standard protein markers, gel loading solution, buffer, FlashBlue™ Protein Stain Powder.


All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, white light visualization system, automatic micropipette with fine tips, microtest tube holder, lab glassware, methanol, glacial acetic acid, distilled or deionized water.


Storage:

Some Components Require Freezer Storage

Resources

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Protein Electrophoresis - In this workshop, Dr. Danielle Snowflack runs a protein electrophoresis experiment from start to finish. While the gel is running, she discusses how proteins are built within a cell and how their structure affects their function.

Additional Resources:

Replenisher

Groups Size:

For 6 groups sharing 3 gels


Time Required:

Electrophoresis - 60 minutes / Staining - 20 minutes / Destaining - 2 hours


Kit Includes:

Instructions, denatured LyphoProtein™ samples, standard protein markers, gel loading solution, buffer, FlashBlue™ Protein Stain Powder.


All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, white light visualization system, automatic micropipette with fine tips, microtest tube holder, lab glassware, methanol, glacial acetic acid, distilled or deionized water.


Storage:

Some Components Require Freezer Storage

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - Biotechnology Basics: Protein Electrophoresis - In this workshop, Dr. Danielle Snowflack runs a protein electrophoresis experiment from start to finish. While the gel is running, she discusses how proteins are built within a cell and how their structure affects their function.

Additional Resources: