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Sickle Cell Gene Detection (DNA-based) with SYBR® Safe

SKU: 116-S
BEST SELLER
$105.00

ABOUT THIS PRODUCT:


Sickle Cell Anemia is a common genetic disease that causes long rods in red blood cells, giving them a "sickled" appearance. These cells get stuck in small capillaries of the blood stream leading to oxygen deprivation that causes pain and organ damage. Sickle Cell Anemia is caused by a single point mutation in the hemoglobin gene that results in a faulty protein. In this experiment, your students will investigate the restriction enzyme that discriminates between HbA (normal) and HbS (disease) genes and perform a simulated test on a patient.

**Includes SYBR® Safe DNA Stain, NOT FlashBlue™ DNA Stain**
• Discover how a single nucleotide mutation can profoundly affect a person’s health
• Explore Mendelian genetics and the molecule methods used to identify sickle cell disease
• Perform agarose gel electrophoresis to separate differently-sized DNA molecules
• Load, run, analyze, and size control samples and samples from a family of three to identify mutations in their hemoglobin genes

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 8 Gels


Time Required:

Complete in 45 minutes


Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.


Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Resources

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - See the EDGE™ in Action! Featuring EDVO-Kit #116 - In this workshop, Dr. Danielle Snowflack discusses our best-selling EDVO-Kit #116, "Sickle Cell Gene Detection" and watch as she runs the full electrophoresis experiment in the EDGE™ Integrated Electrophoresis System (Cat. #500). See the EDGE™ in Action from start to finish!

Safety Data Sheets:

Quick Guides:

Group Size:

For 8 Gels


Time Required:

Complete in 45 minutes


Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.


All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.


Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Instructional Videos:

BioTech Equipment Tips:

Edvotek® YouTube Live Stream - See the EDGE™ in Action! Featuring EDVO-Kit #116 - In this workshop, Dr. Danielle Snowflack discusses our best-selling EDVO-Kit #116, "Sickle Cell Gene Detection" and watch as she runs the full electrophoresis experiment in the EDGE™ Integrated Electrophoresis System (Cat. #500). See the EDGE™ in Action from start to finish!

Safety Data Sheets:

Quick Guides: