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Edvotek at Home

"Edvotek at Home" is a set of resources to teach the basics of Edvotek’s labs through worksheets and presentations. While we believe in the importance of hands-on learning, these free online learning tools are ideal if you can not perform the hands-on experiments in class. Each set includes a student sheet, an instructor’s guide, and an accompanying powerpoint presentation and results sheet. This resource is provided in a downloadable zipped folder below.

Protein Electrophoresis - Proteins produce a unique challenge for electrophoresis because they have complex shapes and different charges, which affect how they migrate through the gel. SDS polyacrylamide-gel electrophoresis, or SDS-PAGE, is a technique that is used to separate proteins according to their molecular weight. The protein samples have been denatured by incubation with a strong detergent, then loaded into a polyacrylamide gel. An electrical current is passed through the gel, pushing the proteins through the gel towards the positive electrode. Since molecules of different sizes travel at different speeds, they separate into discrete “bands” within the gel. In this experiment, SDS-polyacrylamide gel electrophoresis is used to develop an understanding of protein structure, function and diversity.

Presentations

These short courses couple theory with active experimentation to help you update your skills and knowledge in various areas of biotechnology.

Using Biotechnology to Diagnose HIV/AIDS - The Human Immunodeficiency Virus (HIV) causes acquired immune deficiency syndrome (AIDS), a serious disease that suppresses a patient’s immune system, leaving them susceptible to infections. In this simulation, we’ll perform two common tests (western blot, ELISA) used by doctors to diagnose an HIV infection.

A Bright Idea: Using GFP to Teach STEM - Bring exciting STEM learning techniques into your classroom laboratory! In this hands-on workshop, we will build a size-exclusion chromatography column. The column is used to purify green fluorescent protein (GFP) from a crude bacterial extract. Proteins containing fractions are identified by fluorescence and analyzed for purity by SDS-PAGE.

Additional Resources

Instructional Videos - For the visual learner:


Troubleshooting Guide - Frequently asked questions, common mistakes, and how to course correct your experiment:
Quick Guides