PRODUCT UPDATE:
Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.
Group Size:
For 5 plasmid constructs & analyses
Time Required:
Complete 5 modules in approximately 5 hours
Kit Includes:
Instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, SYBR® Safe DNA stain, calibrated pipet
You Need:
Electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, large weigh boats for staining, UV transilluminator or blue light visualization system, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.
Storage:
Some Components Require Freezer & Refrigerator Storage
Instructional Videos:
EdvoTech Tips:
Edvotek Informational Video:
Additional Resources:
Edvotek® YouTube Live Stream - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant - In the lab, we can use molecular tools to cut and paste pieces of DNA from different organisms, allowing us to study gene function or even transform bacteria into living factories! These powerful molecular cloning techniques are used daily in research labs around the world, but simple enough to be performed in your classroom laboratory. In this live stream, Dr. Danielle Snowflack discusses each module of experiment #301, highlights potential pitfalls, and provides tricks for experimental success!
- Video - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant
- Slides - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant
Miscellaneous:
Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.
Group Size:
For 5 plasmid constructs & analyses
Time Required:
Complete 5 modules in approximately 5 hours
Kit Includes:
Instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, SYBR® Safe DNA stain, calibrated pipet
You Need:
Electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, large weigh boats for staining, UV transilluminator or blue light visualization system, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.
Storage:
Some Components Require Freezer & Refrigerator Storage
Instructional Videos:
EdvoTech Tips:
Edvotek Informational Video:
Additional Resources:
Edvotek® YouTube Live Stream - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant - In the lab, we can use molecular tools to cut and paste pieces of DNA from different organisms, allowing us to study gene function or even transform bacteria into living factories! These powerful molecular cloning techniques are used daily in research labs around the world, but simple enough to be performed in your classroom laboratory. In this live stream, Dr. Danielle Snowflack discusses each module of experiment #301, highlights potential pitfalls, and provides tricks for experimental success!
- Video - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant
- Slides - AMA EDVO-Kit #301 Construction & Cloning of a DNA Recombinant
Miscellaneous: