Construction and Cloning of a DNA Recombinant

SKU: 301
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Cloning is frequently performed to study gene structure and function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis. Recommended for college-level courses.

PRODUCT UPDATE: Storage conditions for some components have changed as of 02/17/17. Be sure to download the most recent version of the literature before performing the experiment.

Group size: For 5 plasmid constructs & analyses

Time required: Complete 5 modules in approximately 5 hours

Kit includes: instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, stains, calibrated pipet

You need: electrophoresis apparatus and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtest tubes, pipet pump or bulb, 5 or 10 ml pipets, laboratory glassware, metric rulers, distilled water, ice.

Storage: Some Components Require Freezer & Refrigerator Storage

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