Water Quality Testing III: Multiplex PCR Testing of Water Contaminants

SKU: 953
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$205.00

ABOUT THIS PRODUCT:


Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

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Details

PRODUCT UPDATE:

Experimental components have changed to include new LyphoPrimer™ and LyphoControl™ reagents and SYBR® Safe DNA stain. Please read the accompanying literature as directed in your kit.

Group Size

For 25 students

Time Required:

Extraction - 60 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 mins or overnight

Kit Includes:

Instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.

Storage:

Some Components Require Refrigerator or Freezer Storage Upon Receipt

Resources

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

Replenisher

PRODUCT UPDATE:

Experimental components have changed to include new LyphoPrimer™ and LyphoControl™ reagents and SYBR® Safe DNA stain. Please read the accompanying literature as directed in your kit.

Group Size

For 25 students

Time Required:

Extraction - 60 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 mins or overnight

Kit Includes:

Instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.

Storage:

Some Components Require Refrigerator or Freezer Storage Upon Receipt

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

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