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Discovering Quantitative PCR Amplification and Analysis

SKU: 380
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Quantitative PCR (qPCR also known as real-time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technology has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics. For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands-on learning, and an essential skill for future research work. In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis.

**This kit must be used with a RT Thermal Cycler**

Group size: For 4 lab groups

Time required:

QPCR Setup - 45 minutes
QPCR - 90 minutes
Single Amplification Confirmation by Electrophoresis - 60 minutes
Staining - 5 minutes to overnight

Analysis of Results 45 minsKit includes: instructions, qPCR Master Mix with Fluorescent Dye, Primer Mix, DNA Template, Experimental DNA Samples, Ultra-Pure Water, DNA Standard Marker, UltraSpec-AgaroseTM, 50X Electrophoresis Buffer, 10x Gel Loading Solution, SYBR® Safe Stain, Microcentrifuge & qPCR Tubes.

All you need: qPCR Thermal Cycler, Microcentrifuge, Micropipets: 5-50 μl, Horizontal Gel Electrophoresis Apparatus, DC Power Supply, UV Transilluminator or White Light Box, Microwave or Hot Plate, Pipet Pump, Balance, Hot Gloves, Laboratory Gloves, Beaker, Distilled Water, Ice.

Storage: Some Components Require Freezer Storage Upon Receipt.

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