ChickCheck: The Genetics Behind Blue Chicken Eggs

SKU: 343
NEW
$179.00

ABOUT THIS PRODUCT:


In this experiment, students will investigate the genetic basis of chicken egg color by using PCR to detect a viral insertion near the SLCO1B3 gene, which is responsible for the blue egg color. The PCR products are analyzed using electrophoresis to determine phenotypes and select breeding pairs that will produce homozygous blue egg-laying chickens.

• Explore the genetics of chicken egg color using hands-on PCR.
• Detect a viral DNA insertion near the SLCO1B3 gene that causes blue eggs.
• Compare PCR band patterns to identify egg-color phenotypes.
• Use genetic results to select breeding pairs for homozygous blue egg-laying chickens.

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 25 students working in 5 groups

Time Required:

Set Up - 30-45 minutes
PCR - 2 hours or overnight
Electrophoresis - 45 minutes

Kit Includes:

Instructions, PCR EdvoBeads™ PLUS, LyphoPrimer™ Mix, 100bp Ladder, Control DNA and Chicken DNA, TE Buffer, Universal DNA Buffer, SYBR® Safe DNA Stain, FlashBlue™ DNA Stain, Ultra-Spec Agarose™, TBE Electrophoresis Buffer Powder, 0.2 mL PCR Tubes, Plastic Micropestles, & Snap-top Microcentrifuge Tubes

All You Need:

DNA Electrophoresis Apparatus & Power Supply, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlue™ stain), Microcentrifuge & Microwave or Hot plate, Hot Gloves, Disposable Laboratory gloves , Ice Buckets and Ice, Distilled or Deionized Water, Fertilized Egg (optional for Alternative protocol, Appendix A).

Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Group Size:

For 25 students working in 5 groups

Time Required:

Set Up - 30-45 minutes
PCR - 2 hours or overnight
Electrophoresis - 45 minutes

Kit Includes:

Instructions, PCR EdvoBeads™ PLUS, LyphoPrimer™ Mix, 100bp Ladder, Control DNA and Chicken DNA, TE Buffer, Universal DNA Buffer, SYBR® Safe DNA Stain, FlashBlue™ DNA Stain, Ultra-Spec Agarose™, TBE Electrophoresis Buffer Powder, 0.2 mL PCR Tubes, Plastic Micropestles, & Snap-top Microcentrifuge Tubes

All You Need:

DNA Electrophoresis Apparatus & Power Supply, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlue™ stain), Microcentrifuge & Microwave or Hot plate, Hot Gloves, Disposable Laboratory gloves , Ice Buckets and Ice, Distilled or Deionized Water, Fertilized Egg (optional for Alternative protocol, Appendix A).

Storage:

Some Components Require Freezer Storage Upon Receipt

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