Reeling in the Truth: Identifying Red Snapper using Genetic Analysis

SKU: 341
NEW
$179.00

ABOUT THIS PRODUCT:


In this experiment, students will explore the genetic diversity of fish species in a simulated DNA barcoding experiment. Students will extract DNA from simulated fish samples and use PCR to amplify three gene regions that are conserved between species.

• Learn how to extract DNA from simulated fish samples and amplify specific gene regions using PCR.
• Understand how conserved gene regions can be used to identify species and study genetic diversity.
• Explore DNA barcoding, a critical tool in species identification and conservation biology.

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Details

Group Size:

For 24 reactions, or 6 groups of 4 students

Time Required:

Set Up - 30-45 minutes
PCR - 2 hours or overnight
Electrophoresis - 45 minutes

Kit Includes:

Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, Red Snapper Template, DNA Templates #1-3, TE Buffer, Universal DNA Buffer, Proteinase K, Rice Paper for Simulated Fish Samples, Ultra-Spec Agarose™, 50x Electrophoresis Buffer, FlashBlue™ DNA Stain, SYBR® Safe, 1.5mL Microcentrifuge Tubes, 1.5mL Screw-cap Tubes, 0.2mL PCR Tubes, Petri Plates

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlue™ stain), Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Resources

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

Group Size:

For 24 reactions, or 6 groups of 4 students

Time Required:

Set Up - 30-45 minutes
PCR - 2 hours or overnight
Electrophoresis - 45 minutes

Kit Includes:

Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, Red Snapper Template, DNA Templates #1-3, TE Buffer, Universal DNA Buffer, Proteinase K, Rice Paper for Simulated Fish Samples, Ultra-Spec Agarose™, 50x Electrophoresis Buffer, FlashBlue™ DNA Stain, SYBR® Safe, 1.5mL Microcentrifuge Tubes, 1.5mL Screw-cap Tubes, 0.2mL PCR Tubes, Petri Plates

All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlue™ stain), Microcentrifuge & Microwave or Hot plate.

Storage:

Some Components Require Freezer Storage Upon Receipt

Quick Guides:

Troubleshooting Guides:

Safety Data Sheets:

Instructional Videos:

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