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Mapping of Restriction Sites on Plasmid DNA with SYBR® Safe

SKU: 105-S
$109.00

ABOUT THIS PRODUCT:


DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

• **Includes SYBR® Safe DNA Stain, NOT FlashBlue™ DNA Stain**
• Understand and explore restriction enzymes using DNA mapping
• Perform agarose gel electrophoresis to separate DNA fragments from three restriction enzyme digestions plus a standard marker
• Determine the size of the DNA fragments generated
• Construct a restriction enzyme map of the plasmid

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 8 gels

Time Required:

Complete in 45 minutes

Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Resources

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

Group Size:

For 8 gels

Time Required:

Complete in 45 minutes

Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, SYBR® Safe DNA Stain.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Instructional Videos:

BioTech Equipment Tips:

Safety Data Sheets:

Quick Guides:

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