The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA
Kit includes: instructions, proteinase K, PCR EdvoBeads™, control DNA & primers, microtubes, agarose, DNA ladder, practice gel loading solution, gel loading dye, buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All you need: micropipets to measure between 5 and 50 µl, tips, waterbath, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
Storage: Some Components Require Freezer Storage
Extraction 50 mins PCR Setup 10 mins PCR 2 hours Electrophoresis 60 mins Staining 5 mins to overnight