Mitochondrial DNA Analysis Using PCR
The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA.
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Group size: For 25 students Time required: Extraction - 50 minutes PCR Setup - 10 minutes PCR - 2 hours Electrophoresis - 60 minutes Staining - 5 minutes to overnight Kit includes: Instructions, PCR EdvoBeads™ PLUS, Mitochondrial LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR®Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube. All you need: DNA Electrophoresis, Thermal Cycler, Micropipets: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt