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Mitochondrial DNA Analysis Using PCR 332
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The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA
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Kit includes: instructions, proteinase K, PCR beads, control DNA & primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All you need: micropipettes to measure between 5 and 50 µl, tips, waterbath, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
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