Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
For up to 20 plasmid isolations for up to 12 (7x7cm) gels
Kit includes: instructions, Plasmid LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain, InstaStain® Blue.
You need: electrophoresis apparatus and power supply, waterbath, balance, microcentrifuge, microwave or hot plate, automatic pipet with tips, visualization (white light), misc. labware, 95-100% isopropanol, distilled or deionized water, ice.
Storage: Plasmid LyphoCells™ require freezer storage. Keep all other components at Room Temperature. Note – Kit is shipped ambient.