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Mini-Prep Isolation of Plasmid DNA 202
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Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
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Kit includes: instructions, LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.
You need: electrophoresis apparatus and power supply, waterbath, balance, microcentrifuge, microwave or hot plate, automatic pipet with tips, visualization (white light), misc. labware, 95-100% isopropanol, distilled or deionized water, ice.
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