Restriction Enzyme Mapping
In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.
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Group size: 6 sets of restriction digestions Time required: Complete in 1 hour 20 minutes to 1 hour 45 minutes Kit includes: instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps All you need: DNA Electrophoresis, Micropipets: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate. Storage: Some Components Require Freezer Storage Upon Receipt.