In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.
* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas)
Kit includes: instructions, Hind III & Bgl I restriction enzymes, plasmid DNA, buffers, enzyme grade water, standard DNA fragments, gel loading solution, agarose, electrophoresis buffer, FlashBlue™ Stain.
You need: electrophoresis apparatus and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, floating racks, misc. lab glassware, ice.