Restriction Enzyme Mapping
In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.
6 Sets of Restriction Digestions
Complete in 1 hr. 20 min. to 1 hr. 45 min.
Kit includes: instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer, 10x Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps You need: electrophoresis apparatus and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, floating racks, misc. lab glassware, ice. Storage: Some Components Require Freezer Storage