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Lighting Up Life: Expression of GFP in C. elegans

SKU: 858
$205.00

ABOUT THIS PRODUCT:


Scientists can directly manipulate an organism's genome to produce a phenotype using engineered genes called transgenes. In this experiment, students will use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 10 lab groups


Time Required:

Growing Bacteria - Overnight
Plating Worms - 15 minutes
Worm Growth - 3-4 Days
Set Up - 30 minutes
PCR - 2 hours or overnight
Electrophoresis - 90 minutes


Kit Includes:

Instructions, C. elegans, E. coli OP50 cells, PCR EdvoBeads™ PLUS, GFP primer mix concentrate, EdvoQuick™ DNA ladder, control DNA concentrate, NGM salts, sterile water, TE buffer, extraction buffer, ReadyPour™ NGM agar, petri dishes, pipets, tubes, UltraSpec-Agarose™, electrophoresis buffer (50x), 10x gel loading solution, SYBR® Safe DNA stain, FlashBlue™ liquid stain, sterile loops


All You Need:

Fluorescent and bright field microscopes, thermal cycler or three water baths, electrophoresis apparatus, power supply, balance, microcentrifuge, water bath (55˚C and boiling), UV transilluminator or blue light visualization for SYBR® Safe stained gels, UV safety goggles, white light visualization system (optional if using with FlashBlue™), adjustable micropipettes with tips, microwave or hot plate, pipet pump, covered box or incubator, permanent markers, flasks or beakers, hot gloves, disposable laboratory gloves, distilled or deionized water, sterile water, ice buckets and ice, bleach solution or laboratory disinfectant


Storage:

Some Components Require Refrigerator & Freezer Storage

Replenisher

Group Size:

For 10 lab groups


Time Required:

Growing Bacteria - Overnight
Plating Worms - 15 minutes
Worm Growth - 3-4 Days
Set Up - 30 minutes
PCR - 2 hours or overnight
Electrophoresis - 90 minutes


Kit Includes:

Instructions, C. elegans, E. coli OP50 cells, PCR EdvoBeads™ PLUS, GFP primer mix concentrate, EdvoQuick™ DNA ladder, control DNA concentrate, NGM salts, sterile water, TE buffer, extraction buffer, ReadyPour™ NGM agar, petri dishes, pipets, tubes, UltraSpec-Agarose™, electrophoresis buffer (50x), 10x gel loading solution, SYBR® Safe DNA stain, FlashBlue™ liquid stain, sterile loops


All You Need:

Fluorescent and bright field microscopes, thermal cycler or three water baths, electrophoresis apparatus, power supply, balance, microcentrifuge, water bath (55˚C and boiling), UV transilluminator or blue light visualization for SYBR® Safe stained gels, UV safety goggles, white light visualization system (optional if using with FlashBlue™), adjustable micropipettes with tips, microwave or hot plate, pipet pump, covered box or incubator, permanent markers, flasks or beakers, hot gloves, disposable laboratory gloves, distilled or deionized water, sterile water, ice buckets and ice, bleach solution or laboratory disinfectant


Storage:

Some Components Require Refrigerator & Freezer Storage