Exploring Plant Diversity with DNA Barcoding

Group Size:

For 10 lab groups

Extraction - 2 hours
PCR Setup - 10 minutes
PCR - 2 hours
DNA Digest - 60 minutes
Staining - 5 minutes to overnight

Instructions, PCR EdvoBeads™, Primer G Mix Concentrate, Primer LP Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, NaCl Solution, DNA Extraction Buffer, Restriction Enzyme Reaction Buffer, Restriction Enzyme Dilution Buffer, AluI Restriction Enzyme, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Electrophoresis Buffer, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, Micropestles, Microcentrifuge & PCR Tubes, & Wax Beads.

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate, Scissors or Hole Punch, & Isopropanol.

Some Components Require Freezer Storage Upon Receipt

• #338 FB - EDVO-Kit #338 Replenisher (Comp. B-G, J-L)
• #PCR 25 - EDVO-Kit PCR Bag Replenisher

Quick Guides:

• Polymerase Chain Reaction

Troubleshooting Guides:

• PCR & Electrophoresis Troubleshooting

Safety Data Sheets:

• EDVO-Kit #338 SDS

Instructional Videos:

• Polymerase Chain Reaction
• Electrophoresis
• Visualizing DNA