DNA Fingerprinting by Southern Blot

SKU: 311
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In this experiment, students gain experience in non-isotopic DNA detection & the use of Southern Blot analysis in DNA fingerprinting for a hypothetical paternity test. Includes three modules: agarose gel electrophoresis, Southern Blot transfer, and non-isotopic detection of DNA.

* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas)

Group size: For 5 lab groups

Time required: Electrophoresis - 45 minutes | Blot - overnight | Non-Isotopic Detection - 3-4 hours

Kit includes: instructions, predigested DNA samples, buffers, NBT/BCIP tablets, streptavidin-Alkaline Phosphatase, nylon membranes, filter paper, UltraSpec-Agarose™ powder.

You need: electrophoresis apparatus & power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, incubation oven, pipet pumps or bulbs, pipets, floating Racks for microtest tubes, lab glassware, plastic wrap, distilled or deionized water, NaCl, NaOH, Concentrated HCl, ice.

Storage: Some Components Require Refrigerator & Freezer Storage.

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