DNA Fingerprinting by Southern Blot

SKU: 311
$349.00

ABOUT THIS PRODUCT:


In this experiment, students gain experience in non-isotopic DNA detection & the use of Southern Blot analysis in DNA fingerprinting for a hypothetical paternity test. Includes three modules: agarose gel electrophoresis, Southern Blot transfer, and non-isotopic detection of DNA.

* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas)

DOWNLOAD SAMPLE INSTRUCTIONS
Details

Group Size:

For 5 lab groups

Time Required:

Electrophoresis - 45 minutes | Blot - overnight | Non-Isotopic Detection - 3-4 hours

Kit Includes:

Instructions, predigested DNA samples, buffers, NBT/BCIP tablets, streptavidin-Alkaline Phosphatase, nylon membranes, filter paper, UltraSpec-Agarose™ powder.

You Need:

Electrophoresis apparatus & power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, incubation oven, pipet pumps or bulbs, pipets, floating Racks for microtest tubes, lab glassware, plastic wrap, distilled or deionized water, NaCl, NaOH, Concentrated HCl, ice.

Storage:

Some Components Require Refrigerator & Freezer Storage

Replenisher:

Resources

Download EDVO-Kit #311 Safety Data Sheets (SDS)

Group Size:

For 5 lab groups

Time Required:

Electrophoresis - 45 minutes | Blot - overnight | Non-Isotopic Detection - 3-4 hours

Kit Includes:

Instructions, predigested DNA samples, buffers, NBT/BCIP tablets, streptavidin-Alkaline Phosphatase, nylon membranes, filter paper, UltraSpec-Agarose™ powder.

You Need:

Electrophoresis apparatus & power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, incubation oven, pipet pumps or bulbs, pipets, floating Racks for microtest tubes, lab glassware, plastic wrap, distilled or deionized water, NaCl, NaOH, Concentrated HCl, ice.

Storage:

Some Components Require Refrigerator & Freezer Storage

Replenisher:

Download EDVO-Kit #311 Safety Data Sheets (SDS)
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