Bacterial Protein Fingerprinting

SKU: 252
$60.00

ABOUT THIS PRODUCT:


The objective of this experiment is for students to prepare soluble protein lysates from several species of bacteria and analyze the electrophoretic protein profiles to identify an unknown.

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Details

Group Size:

For 6 groups sharing 3 gels


Time Required:

Day One Set Up - 15 minutes / Day Two Set Up - 60 minutes / Electrophoresis - 60 minutes / Staining - 20 minutes / Destaining - 2 hours


Kit Includes:

Instructions, bacterial cultures and reagents, Lysozyme, buffers, Protein InstaStain®, practice gel loading solution, protein sample buffer, unknown proteins ready for electrophoresis, ReadyPour™ Agar, nutrient broth, screw cap tubes, sterile inoculating loops, sterile 1 ml pipets, sterile petri plates, 100 x 15 mm


All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, microcentrifuge, incubation oven, hot plate or burner, white light visualization system, automatic micropipette with fine tips, lab glassware, methanol, glacial acetic acid, distilled water, sterile water or nutrient broth, plastic wrap, photodocumentation system (optional)


Storage:

Some Components Require Freezer Storage

Resources

Quick Guides:

Troubleshooting Guide:

Safety Data Sheets:

Instructional Videos:

Replenisher

Group Size:

For 6 groups sharing 3 gels


Time Required:

Day One Set Up - 15 minutes / Day Two Set Up - 60 minutes / Electrophoresis - 60 minutes / Staining - 20 minutes / Destaining - 2 hours


Kit Includes:

Instructions, bacterial cultures and reagents, Lysozyme, buffers, Protein InstaStain®, practice gel loading solution, protein sample buffer, unknown proteins ready for electrophoresis, ReadyPour™ Agar, nutrient broth, screw cap tubes, sterile inoculating loops, sterile 1 ml pipets, sterile petri plates, 100 x 15 mm


All You Need:

3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, microcentrifuge, incubation oven, hot plate or burner, white light visualization system, automatic micropipette with fine tips, lab glassware, methanol, glacial acetic acid, distilled water, sterile water or nutrient broth, plastic wrap, photodocumentation system (optional)


Storage:

Some Components Require Freezer Storage

Quick Guides:

Troubleshooting Guide:

Safety Data Sheets:

Instructional Videos: