The BiotechnologyEducation Company
What are BactoBeads™?
BactoBeads™ are non-pathogenic, freeze-dried microbes (bacteria and lower eukaryotes) that are easily cultured for use in the classroom. Each bead contains microorganisms, buffer, salts and nutrient broth in an instantly soluble pellet.
Tagged on: Tips and Tricks, BactoBeads, Transformation
Taking Transformation to the Next Level With Colony PCR
Transformation is a biotechnology technique that is used daily in research laboratories around the world. This technique allows scientists to force E. coli to take up small, circular pieces of DNA called plasmids from the surrounding environment. Using recombinant DNA technology, these plasmids are engineered to contain genes from different sources.
Tagged on: GFP, PCR, Transformation, New, Classroom Laboratory
DNA Ligase - Building a Bridge with DNA
DNA ligase is one of the most important, but least celebrated, enzymes used in modern biotechnology. This enzyme plays a very important role in DNA replication and repair by acting like a “glue” that can connect different DNA fragments to one another. Scientists take advantage of this molecular glue to combine DNA fragments to make an entirely new sequence. However, many people are merely using DNA ligase as a tool without knowing the story and mechanism behind it.
Tagged on: Beginner, Biotech Basics, DNA, Genes, Plasmids, Restriction Digest, Restriction Endonucleases, Restriction Enzymes, STEM, Transformation, Ligation, DNA Ligase, Recombinant DNA Technology
Five Ways to Improve Your Transformation Experiments
Transformation is the process through which bacteria can pick up foreign DNA from the surrounding environment. The newly acquired genetic information is both stable and heritable. In the laboratory, we can transform bacteria with small, circular pieces of DNA called plasmids. These plasmids are engineered to contain genes from different sources. Once transformed into bacteria, the plasmids turn the bacteria into living factories to create medications, vitamins and other useful products.
Tagged on: Biotech Basics, Science, Tech Tips, Tips and Tricks, Classroom Laboratory, Transformation, Genetic Engineering, Recombinant DNA Technology, Plasmids
Lighting Up Life With GFP
Meet the luminescent Aequorea victoria. This jellyfish lights up the eastern Pacific Ocean with a green fluorescent signal. Producing this signal is a two steps process. First a blue light is created through an interaction between released calcium (Ca2+) and the protein aeqorin. Then the blue light is turned green by a protein known as Green Fluorescent Protein (GFP). Both proteins have become powerful tools in biotechnology.
Tagged on: Genes, GFP, Transformation, Cell
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