The BiotechnologyEducation Company
Taking Transformation to the Next Level With Colony PCR
Transformation is a biotechnology technique that is used daily in research laboratories around the world. This technique allows scientists to force E. coli to take up small, circular pieces of DNA called plasmids from the surrounding environment. Using recombinant DNA technology, these plasmids are engineered to contain genes from different sources.
Tagged on: GFP, PCR, Transformation, New, Classroom Laboratory
Lighting Up Life With GFP
Meet the luminescent Aequorea victoria. This jellyfish lights up the eastern Pacific Ocean with a green fluorescent signal. Producing this signal is a two steps process. First a blue light is created through an interaction between released calcium (Ca2+) and the protein aeqorin. Then the blue light is turned green by a protein known as Green Fluorescent Protein (GFP). Both proteins have become powerful tools in biotechnology.
Tagged on: Genes, GFP, Transformation, Cell
Biotech Basics: A Plasmid Primer
In 1952, Nobel Prize Winner Joshua Lederberg introduced the term ‘plasmid’, a double-stranded, circular DNA molecule that naturally exists in bacterial cells and some eukaryotes. Plasmids have been used by molecular biologists to study, analyze, and clone genes. They are capable of self-replicating and are distinct from the cells’ chromosomal DNA.
Tagged on: Bacterial Cell, Biotech Basics, Circular DNA Molecule, DNA, FAQ, GFP, Plasmids, Plasmid Preparation, Tech Tips, Transformation
School’s Out for Summer…Or Is It?
The 2013-14 school year is wrapping up, and we’re sure that you (and your students) are anxiously counting down the minutes. However, whereas your students will get to leave all thoughts of class behind for the summer, we know you’ll be working to set up new classes or trying to incorporate new experiments into your existing curriculum.
Tagged on: Advanced, Intermediate, Biotech Basics, FAQ, Genes, GFP, Mutation, Polymerase Chain Reaction, Tech Tips, Transformation
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