The BiotechnologyEducation Company
DNA Sequencing to Save Endangered Species
Since the 1980’s, DNA evidence has been used in the criminal justice system to help convict violent offenders and, just as importantly, exonerate innocent individuals. An approach often used is DNA profiling, a technique where investigators compare the base pair order of DNA found at the crime scene to a suspect’s DNA. Now, another type of genetic analysis – DNA barcoding – is being used to combat illegal wildlife trading.
Tagged on: DNA, Barcoding, Wildlife, Forensics, Science, Advanced
DNA Ligase - Building a Bridge with DNA
DNA ligase is one of the most important, but least celebrated, enzymes used in modern biotechnology. This enzyme plays a very important role in DNA replication and repair by acting like a “glue” that can connect different DNA fragments to one another. Scientists take advantage of this molecular glue to combine DNA fragments to make an entirely new sequence. However, many people are merely using DNA ligase as a tool without knowing the story and mechanism behind it.
Tagged on: Beginner, Biotech Basics, DNA, Genes, Plasmids, Restriction Digest, Restriction Endonucleases, Restriction Enzymes, STEM, Transformation, Ligation, DNA Ligase, Recombinant DNA Technology
Visualizing DNA After Agarose Gel Electrophoresis
Electrophoresis is a technique that allows us to separate DNA, RNA or proteins into bands according to their size. For more information about this technique, be sure to check out our previous post describing electrophoresis.
Tagged on:Biotech Basics, Science, Technology, Electrophoresis, Classroom Laboratory, DNA, STEM, DNA Fragments, Beginner, Intermediate
Why is C. elegans So Popular in the Biology Laboratory?
For centuries, scientists have used model organisms from the three domains of life, bacteria, archaea, and eukaryotes, to understand biological functions. For example, the bacterium Escherichia coli, which has been studied for more than 60 years, has provided important findings in the areas of biological engineering and industrial microbiology. On the other hand, eukaryotic domain organisms like the fly Drosophila melanogaster and the roundworm Caenorhabditis elegans (C. elegans) have been widely used to study genetics and developmental biology.
Tagged on: Biotech Basics, DNA, STEM, Beginner, Intermediate, Advanced, DNA Sequencing, Genes, Mutation, NSTA, Organisms, Workshop
The Evolution of Electrophoresis
Agarose gel electrophoresis is a biotechnology technique used to separate DNA molecules according to their size. The mixture of molecules is added into depressions (or “wells”) within a gel, and then an electrical current is passed through the gel. Because the sugar-phosphate backbone of DNA has a strong negative charge, the current drives the DNA through the gel towards the positive electrode.
Tagged on: Biotech Basics, Science, Technology, Electrophoresis, Classroom Laboratory, DNA, STEM, DNA Fragments, Beginner, Intermediate
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