This experiment has been adapted from a freshwater quality test which uses Daphnia magna to determine toxicity levels of freshwater. A simulated "toxicant" is provided to simulate environmental pollution in freshwater lakes, rivers and streams. Hydrolysis of a fluorescent substrate by Daphnia is used to determine the level of toxicants. Results are observed by using long wave ultraviolet light. Calculations for lethal concentration are determined.
You need: Daphnia magna, 4 additional molded exposure chambers (Cat. #965), long wave UV light, white light visualization system, microscope or hand magnifier, large glass vessel, beakers, UV protective goggles, spring water, test tubes, 5 ml pipets and pumps.