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Water Quality Testing III: Multiplex PCR Testing of Water Contaminants 953
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Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
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Kit includes: instructions, control DNA and primers, DNA ladder, nonpathogenic E. coli, B. subtilis, S. marcenscens, proteinase K, PCR beads, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
All You need: Automatic micropipettes (5 - 50 µl) and tips, waterbath, ice, balance, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.
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